Thermal Characterization of Purified Glucose Oxidase from A Newly Isolated Aspergillus Niger UAF-1

Purification and Properties of the Glucose Oxidase from Aspergillus Niger.

Glucose oxidase (/3-n-glucose : 02 oxidoreductase, EC 1.1.3.4) has been highly purified from the extracts of three fungi, Penicillium not&urn (1, 2), Penicillium amagasakiense (3, 4), and Aspergillus niger (5, 6). It has also been identified and sometimes partially purified from a number of other sources. Some of these sources are reviewed by Schepartz and Subers (7). Recently Pazur and Kleppe ...

Aspergillus terreus Unimap AA-1: A Newly Isolated Extracellular Glucose Oxidase-Producing Strain

Glucose oxidase (GOx) has found wide range of industrial applications. Commercial GOx are produced from Aspergillus niger and selected strains of Penicllium species, however, these strains are associated with some drawbacks. Aspergillus niger produces predominantly intracellular GOx, which requires extra recovery processes thus incurs more processing cost while Penicillium sp. exhibits filament...

Irreversible thermal denaturation of Glucose oxidase from Aspergillus niger

Glucose oxidase from Aspergillus niger: Production, characterization and immobilization for glucose oxidation

Glucose oxidase (GOx), a flavoenzyme, from Aspergillus niger was produced, purified and immobilized for glucose oxidation. Maximum activities of 0.6 and 0.31U mg fungus dry weight were obtained as intracellular and extracellular respectively. Optimal production (4000 IU/L) was achieved when basal salt medium was supplemented with sucrose (7.5%), peptone (1.5%) phosphorus (0.2%) and MgSO4 (0.2%)...

Oxalic acid production by Aspergillus niger: an oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese.

The external pH appeared to be the main factor governing oxalic acid production by Aspergillus niger. A glucose-oxidase-negative mutant produced substantial amounts of oxalic acid as long as the pH of the culture was 3 or higher. When pH was decreased below 2, no oxalic acid was formed. The activity of oxaloacetate acetylhydrolase (OAH), the enzyme believed to be responsible for oxalate formati...